The topic of the EFB lesson was ' to prepare your own antibody':

One of the two suggestions was to find a natural source for these antibodies.

The first step is to force cells to produce antibodies. This can be made by injecting antigens into an organism e.g. Mouse:

B-Cells produce antibodies.

Isolate a single B-cell and cultivate the B-cell

And finally to purify these antibodies.

Second possibility: Buy the antibodies.

But how to prepare an antigen?

To solve this problem our class has developed a scheme:

• Isolate the cDNA coding for hCG by PCR
• Clone it into an expression vector
• Produce competent cells (e.g. E.coli or S.cerevisiae)
• Transform the cells
• Induce expression of hCG
• Purify the protein